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KMID : 0379120050330010011
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Korean Journal of Mycology
2005 Volume.33 No. 1 p.11 ~ p.17
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Effect of Various Plant Extracts on the Mycelial Growth and Fibrinolytic Activity of Armillaria mellea
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Choi Han-Suk
Kim Myung-Kon
Park Hyo-Suk
Kim Jae-Sung
KIm Sung-Jun
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Abstract
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To increase the fibrinolytic activity and production of mycelium, extracts of 7 plant species were supplemented to the growth media of Armillaria mellea, and mycelial growth and enzymatic activity in the mycelium extracts of A. mellea were estimated. The mycelial production of A. mellea was slightly increased by adding ASH-R, UDVN or RGR extract, whereas KG extract significantly affected the growth. Supplement of ASH-S, UDVN and RGR extracts increased proteolytic activity from 36.8 to 46.1% Fibrinolytic activity was increased to $50{\sim}65%$ by supplement with RVS, ASH-S and RGR extracts, respectively. Enzyme extracts of the fungus grown with RGR extract supplement degraded all chains of fibrinogen within 2 hours, whereas control was required 3 hours. Degradation of fibrin fragments by the enzyme extracts was also observed through microscopy.
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KEYWORD
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Armillaria mellea, Fibrinolysis, Mycelium, Plant extract, Proteolysis
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